Susceptibility of chalkrood fungus, Ascosphaera apis, isolated from diseased honey bee, Apis mellifera, to varying concentrations of selected food grade preservatives

Date

2011

Degree

Bachelor of Science in Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Arlene L. Llamado

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Abstract

A total of 20 mold isolates were obtained from diseased honey bees collected from apiaries in Laguna and Metro Manila. Characterization of the isolates based on spore and fruiting body formation revealed that the isolates from Laguna had an average spore cyst diameter of 74.0 μm with spore ball diameter of 11.9 μm. The spore cyst and spore ball diameter of Metro Manila isolates, were 72.2 μm and 12.2 μm, respectively. Based on obtained characteristics, all the isolates were identified as Ascosphaera apis. Using the cylinder cup assay, the sensitivity of the isolates to the different food grade preservatives, namely, calcium propionate, potassium sorbate and sodium benzoate was determined. Results showed that calcium propionate had no effect on the organism while the other two had varying degrees of growth inhibition of the isolates based on their concentration. Significant effect was obtained at 1% concentration of the preservatives. Comparison of the activity of the two preservatives revealed that sodium benzoate is less active in inhibiting the growth of Ascosphaera apis than potassium sorbate.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Document Type

Thesis

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