Comparison of spectrophotometric and digital photometric methods for the determination of nitrite in wastewater from meat processing plants

Date

2011

Degree

Bachelor of Science in Chemistry

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Joy Elaine P. Kaligayahan

Co-adviser

Ernesto J. Del Rosario

Committee Member

Marlon N. Manalo

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Abstract

Nitrite concentrations of wastewater from meat processing plants were determined and quantified using digital photometry. This method used a digital camera, a light box, RGB Analysis of Colors software, and a computer. Three equations were used for the determination namely percent luminance equation, a*(red-green component) equation, and b*(yellow-blue component) equation. The method used for the determination of nitrite makes use of the diazotization reaction of sulfanilamide and N- (1-naphthyl)-ethylenediamine. The conventional spectrophotometric method for the determination of nitrite was also done in order to validate the results of the digital photometric method. The mean concentrations of nitrite obtained in the samples using the spectrophotometric method was comparable to the results obtained from % luminance, a*, and b* equations at 95 % confidence level. In the second experiment done, the results obtained from the spectrophotometric method was also comparable to the results obtained from % luminance, a*, and b* equations. Furthermore, the mean nitrite concentrations gathered from the first experiment were comparable to the results obtained from the second experiment at 95 % confidence level. The standard deviations obtained from the spectrophotometric method were also compared with the standard deviations from % luminance, a*, and b* equations and results generally showed that the nitrite concentrations obtained do not differ significantly from each other at 95 % confidence level. Assessment of the repeatability, accuracy, and precision showed that the digital photometric method can be repeatable, accurate, and precise. Mean nitrite concentrations obtained from the samples ranged from 0.1853- 0.9829 mg/L. Wastewater from the meat processing plants that were analyzed did not exceed the maximum contaminant limit of 10 mg/L set by the US Environmental Protection Agency.

Language

English

LC Subject

Spectrophotometry, Nitrites

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

LG 993.5 2011 C4 R33

Document Type

Thesis

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