Calcium pectinate encapsulation of rice bran phytosterols for photodegradative protection and efficient delivery

Date

10-2013

Degree

Bachelor of Science in Chemistry

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Evelyn B. Rodriguez

Restrictions

Restricted: Not available to the general public. Access is available only after consultation with author/thesis adviser and only to those bound by the confidentiality agreement.

Abstract

Phytosterols, the major components of the unsaponifiable fraction of rive bran oil (RBO) are gaining attention owing to their enormous applications in nutraceutical and cosmeceutical industries. In this study, rice bran phytosterols were encapsulated into calcium pectinate microparticles via ionic gelation for photodegradative protection and efficient delivery. The calcium pectinate ionic gelation technique afforded an encapsulation efficiency of 82.96 ± 0.57% and loading efficiency of 13.55 ± 0.09%. The average size of the phytosterol-loaded calcium pectinate microparticle was 766.7 ± 88.19 µm as measured from optical microscopy. The microparticles were found to be stable under refrigerated condition after two months of storage (99.19 ± 0.06%). On exposure to UV radiation (366nm), the phytosterol degradation was 10.74 ± 0.31% and 3.31 ± 0.50% for the non-encapsulated and encapsulated phytosterols, respectively . Assessment of the occlusive property showed that encapsulated phytosterols exhibited a 1.3 fold increase in skin hydration potential than the non-encapsulated phytosterols. Swelling studies revealed microparticle shrinking in simulated gastric fluid (SGF) and swelling in simulated intestinal fluid (SIF). The in vitro releasing profile using an oral delivery model showed that 37.46 ± 3.48% and 91.29 ± 0.24 pf phytosterols were released in SGF and SIF, respectively, after 4 hours. These results indicated that encapsulation protected the phytosterols from the acidic environment of the stomach but allowed an easy release in the intestines where absorption should take place. In vitro releasing study using a skin delivery model showed an initial burst release during the first hour (66.01 ± 0.00%) followed by a slow controlled release until the 24th hour (82.48 ± 2.72%). Using the duck embryo CAM irritation assay, the encapsulated phytosterols exhibited a two-fold higher anti-inflammatory activity than the non-encapsulated phytosterols.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

LG 993.5 2013 C42 /C35

Document Type

Thesis

This document is currently not available here.

Share

COinS