Partial charaterization of acetonitrile extract from soursop (Anona muricata L.) seeds and evaluation of its antioxidative properties

Date

10-2013

Degree

Bachelor of Science in Chemistry

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Hidelisa P. Hernandez

Abstract

This study was conducted to characterize the acetonitrile extract from soursop (Annona muricata L.) seeds and to evaluate its antioxidative properties. The soursop seeds, removed from fruits gathered from Bitin, Laguna, were extracted using acetonitrile as solvent. The acetonitrile extract from soursop seeds showed a positive result from copper acetate test indicating the presence of diterpenes. It also showed positive results to ferric chloride test and lead acetate test signifying the presence of phenolic compounds. Moreover, it gave positive results to Molisch?s test and Liebermann-Burchard test indicating the presence of carbohydrates and sterols, respectively. An eluate collected from column chromatography was subjected to UV-Visible and 1H Nuclear Magnetic Resonance Spectroscopy. The UV-Vis spectrum showed peaks characteristic to compounds containing conjugated alkene, carbonyl and phenol chromophores while the 1H NMR spectrum showed peaks characteristic to compounds having saturated, allylic, vinylic and alcoholic protons.The antioxidative property of the acetonitrile extract from soursop seeds was evaluated using different assays measurement scavenging activity against DPPH, hydrogen peroxide, and hydroxyl radicals, and measurement of iron chelating activity. It did not exhibit a strong iron chelating activity (36.61 ± 0.17 %) at 8000 µg/mL, but showed a high scavenging activity against DPPH (82.33 ± 0.16 %) although lower than that of ascorbic acid (98.20 ± 0.04 %) at 187.50 µg/mL. It also showed high scavenging activity against hydrogen peroxide (90.87 ± 1.31 %) at 8000 µg/mL, and hydroxyl radicals (69.66 ± 0.40 %) at 4000 µg/mL. With these results at hand, it can be concluded that the seeds of soursop contain compounds which possess antioxidative properties, and can therefore be a natural alternative source of antioxidants.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

LG 993.5 2013 C42 /G33

Document Type

Thesis

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