Isolation of free-living nitrogen-fixing bacteria from horse manure vermicompostand screening for growth promoting activity on mungbean (Vigna radiata L.)

Date

2011

Degree

Bachelor of Science in Biology

Major Course

Major in Microbiology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Arlene L. Llamado

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Abstract

Freshly harvested vermicompost, mature vermicompost, and roughage material were collected from Kahariam Farms, Inc. From six vermicompost samples, a total of 17 bacterial isolates were obtained. Isolates exhibited colorless, circular, convex, watery

colonies on N-free medium after the 48-hour incubation period. All isolates were Gram- negative, obligately aerobic cocci to short rods. Presumptive identification of the isolates

was done using the scheme presented in Bergey’s Manual of Systematic Bacteriology. All bacterial isolates exhibited cyst formation, non-motility, and brown soluble pigmentation which are typical characteristics of Azotobacter nigricans. The effect of the putative A. nigricans as inoculant and as soil and vermicompost supplement on the germination and growth of mungbean was determined through plant assay method and pot experimentation, respectively. The results showed that the use of A. nigricans had a positive effect on the germination index and shoot elongation of mungbean. Statistical analyses revealed the use of A. nigricans as inoculants and supplements to vermicompost and soil had a significant effect on mungbean seed germination. However, in terms of shoot elongation, all treatments were not significantly different from the uninoculated control (P=0.05).

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Document Type

Thesis

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