Isolation of free-living nitrogen-fixing bacteria from horse manure vermicompostand screening for growth promoting activity on mungbean (Vigna radiata L.)
Date
2011
Degree
Bachelor of Science in Biology
Major Course
Major in Microbiology
College
College of Arts and Sciences (CAS)
Adviser/Committee Chair
Arlene L. Llamado
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Abstract
Freshly harvested vermicompost, mature vermicompost, and roughage material were collected from Kahariam Farms, Inc. From six vermicompost samples, a total of 17 bacterial isolates were obtained. Isolates exhibited colorless, circular, convex, watery
colonies on N-free medium after the 48-hour incubation period. All isolates were Gram- negative, obligately aerobic cocci to short rods. Presumptive identification of the isolates
was done using the scheme presented in Bergey’s Manual of Systematic Bacteriology. All bacterial isolates exhibited cyst formation, non-motility, and brown soluble pigmentation which are typical characteristics of Azotobacter nigricans. The effect of the putative A. nigricans as inoculant and as soil and vermicompost supplement on the germination and growth of mungbean was determined through plant assay method and pot experimentation, respectively. The results showed that the use of A. nigricans had a positive effect on the germination index and shoot elongation of mungbean. Statistical analyses revealed the use of A. nigricans as inoculants and supplements to vermicompost and soil had a significant effect on mungbean seed germination. However, in terms of shoot elongation, all treatments were not significantly different from the uninoculated control (P=0.05).
Language
English
Location
UPLB Main Library Special Collections Section (USCS)
Recommended Citation
Tolentino, Mark Andrew D., "Isolation of free-living nitrogen-fixing bacteria from horse manure vermicompostand screening for growth promoting activity on mungbean (Vigna radiata L.)" (2011). Undergraduate Theses. 2222.
https://www.ukdr.uplb.edu.ph/etd-undergrad/2222
Document Type
Thesis