Nanoadsorbents from rice hull : characterization and performance evaluation for the isolation of curcin and lysozyme
Date
10-2012
Degree
Bachelor of Science in Chemistry
College
College of Arts and Sciences (CAS)
Adviser/Committee Chair
Marivic S. Lacsamana
Abstract
Silica was extracted from rice hull through acid leaching with 1 M HCl and calcination at 600 °C for 6 hrs. The percent yield of silica was 22.55 ± 0.04 %. MCM-41 (RHSMCM-41) was then prepared at a percentage yield of 86.67 ± 5.34 % using the silica extracted from rice hull (RHS). Using scanning electron microscopy, the average particle size of RHS and RHSMCM-41 were 91.74 ± 18.01 nm and 92.99 ± 22.61 nm, respectively. Elemental dispersive X-ray analysis showed that RHS and RHSMCM-41 contained Si at 41.13 % and 40.76 %, respectively. FTIR spectra of both RHS and RHSMCM-41 showed the presence of silanol and siloxane groups. Adsorption studies using RHS and RHSMCM-41 as nanoadsorbents and bovine serum albumin (BSA) as model protein showed the optimum pH for adsorption to be pH 4.5 and optimum BSA concentration to be 500 ppm, for both nanoadsorbents. In the adsorption studies for the target proteins, curcin and lysozyme, conducted at their respective isoelectric point, RHS showed higher adsorption capacity for curcin and lysozyme than RHSMCM-41. RHS was also able to retain more of the adsorbed proteins compared to RHSMCM-41 suggesting its potential use in immobilization studies.
Language
English
Location
UPLB Main Library Special Collections Section (USCS)
Call Number
LG 993.5 2012 C42 /L33
Recommended Citation
Lacsao, Jeela Florence A., "Nanoadsorbents from rice hull : characterization and performance evaluation for the isolation of curcin and lysozyme" (2012). Undergraduate Theses. 2226.
https://www.ukdr.uplb.edu.ph/etd-undergrad/2226
Document Type
Thesis