Extraction, partial characterization, and assessment of antioxidant activity of the lipophilic extract of Sargassum polycystum

Date

10-2013

Degree

Bachelor of Science in Chemistry

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Hidelisa P. Hernandez

Abstract

Abstract: The lipophilic extract of Sargasssum polycystum was fractionated by liquid column chromatography and characterized by phytochemical screening tests, UV-Vis spectrophotometry, and 1H NMR spectroscopy. Sargassum polycystum samples were collected from Matabungkay, Batangas. The samples were air dried, ground, and extracted using 2:1 chloroform-methanol solution. The extraction yielded 2.90 g of crude extract with 2.84% yield in dry weight. The crude extract was then subjected to liquid column chromatography. The fractions obtained were further purified then characterized along with the crude extract by phytochemical screening tests, UV-Vis spectrophotometry, and 1H NMR spectroscopy. Results showed that the fractions contained only alkene and alcohol functional groups. The antioxidant activity of the crude extract was then determined by DPPH radical scavenging assay, hydrogen peroxide scavenging assay, hydroxyl radical scavenging assay, and ferrous ion chelating activity. Results showed SC50 values of 404, 828, 410.9 ppm for the DPPH radical scavenging assay, hydrogen peroxide radical, and hydroxyl radical scavenging assays, respectively. No significant chelating activity was observed. The obtained SC50 values were then compared against ascorbic acid. Results showed the extract exhibited lower antioxidant activity against the standard. The crude extract did not exhibit antifungal activity against A.niger and A. flavus isolated from cacao seeds.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

LG 993.5 2013 C42 /B36

Document Type

Thesis

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