Parametric study on chemical and enzymatic hydrolosis of sodium alginate from Sargassum kushimotense Yendo (Phaeophyta) for hydrolysates production

Date

2011

Degree

Bachelor of Science in Chemical Engineering

College

College of Engineering and Agro-Industrial Technology (CEAT)

Adviser/Committee Chair

Rex B. Demafelis

Co-adviser

Milagrosa R. Martinez-Goss, Jessica F. Simbahan, Irene G. Pajares

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Abstract

Sargassum kushimotense, a brown macroalga, was used as a feedstock for hydrolysates production. Hydrolysis methods were first evaluated using commercial alginic acid and the method which yielded the highest amount of uronic acid and reducing sugar was applied to the extracted alginic acid. Two types of hydrolysis were employed namely acid and enzymatic. The parameters considered for the acid hydrolysis were reaction temperature, acid concentration, and reaction time. For the enzymatic hydrolysis incubation temperature and incubation time considered. For the acid hydrolysis, the sample subjected to 80% formic acid and heated to 60°C for 1 hour yielded the highest amount of reducing sugar with 2.5963 mg/mI and uronic acid concentration of 1.4796mg/mI. Enzymatic hydrolysis incubated at 37°C for 72 hours yielded the highest reducing sugar concentration of 1.04182mg/mI. The acid hydrolysis condition applied to the extracted alginate produced a reducing sugar concentration of 0.0423mg/mI. For the enzymatic hydrolysis of the extracted alginic acid, 0.5564mg/mI of reducing sugar was produced. With that, potential methods for the hydrolysis of alginic acid fro Sargassum kushimotense were developed.

Language

English

LC Subject

Parametric study, Hydrolysis

Location

UPLB College of Engineering and Agro-Industrial Technology (CEAT)

Call Number

LG 993.5 2011 E62 C38

Document Type

Thesis

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