Inheritance of TY-3 gene for resistance to tomato leaf curl Philippine virus (ToLCPV) in F2 population of tomato (Solanum lypersicum L.)

Date

4-2014

Degree

Bachelor of Science in Agricultural Biotechnology

College

College of Agriculture and Food Science (CAFS)

Adviser/Committee Chair

Alma O. Canama

Abstract

Abstract: Tomato leaf curl disease is a threat in the tomato industry. The Ty-3 gene has been identified to be effective against the tomato leaf curl Philippine virus (ToLCPV). Consequently, studies about Ty-3 gene are conducted in order to fully utilize it in the development of local tomato variety resistant to ToLCPV. The study evaluated the disease response of tomato F2 population at 2, 4, and 6 weeks post inoculation (wpi) derived from the cross between susceptible Super Apollo tomato and resistant tomato line CLN3125 containing Ty-3 gene introgression. A co-dominant SCAR marker (P6-25) was used for the Ty-3 gene specific marker analysis. Results indicated that a moderate resistance disease reaction was observed in the F2 population and a moderate disease progress as measured by area under disease progress curve. The disease reaction was differentiated at 4 wpi. The F2 progenies showed varying reactions to the virus ranging from highly resistant to susceptible disease response. The inheritance of Ty-3 gene-specific marker fitted the expected 1:2:1 Mendelian ratio for an F2 segregating population. A total of 79 F2 progenies were identified as homozygous resistant. The findings of the study significantly contribute to the understanding of the inheritance of the Ty-3 resistance locus in the current tomato breeding efforts for ToLCPV resistance.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

LG 993.5 2014 A127 /C36

Document Type

Thesis

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