The isolation and molecular characterization of Ghrelin gene in Philippine native pigs (Sus scrofa)
Date
4-2014
Degree
Bachelor of Science in Agricultural Biotechnology
College
College of Agriculture and Food Science (CAFS)
Adviser/Committee Chair
Severino S. Capitan
Abstract
Philippine Native pigs are known best for Lechon, which commands a good price for its meat quality, leaner and tastier than imported ones. In swine, one of the potential molecular markers for meat quality is the Ghrelin gene (GHRL), primarily known for its Growth Hormone (GH) - releasing activities and energy homeostasis. This study was conducted to characterize the GHRL gene of Philippine native pigs. Blood and tissue DNA were used for PCR amplification, and then direct sequencing was done to obtain the sequences of the Ghrelin of Philippine native pigs. Blast-N analysis showed that the obtained sequences are highly homologous (99-100%) to other Ghrelin sequences in the NCBI database. A nucleotide polymorphism was found in intron 1, from 143 to 357 base pair among the comparison of Philippine native pig samples and 5 nucleotide polymorphisms were found at the same intron region of Ghrelin of Philippine native pig when compared to the Ghrelin sequences of other swine breeds. It was expected that the nucleotide polymorphisms in the sequence will not alter the amino acid sequence of Ghrelin of the Philippine native pigs because they were found at the intron region of Ghrelin. No polymorphisms were found in the Ghrelin protein sequence among the comparison of Philippine native pig samples and when Philippine native pig samples when compared to the Ghrelin protein sequences of other swine breeds. However, the other regions of Ghrelin DNA sequence should be sequenced and analyzed to find more polymorphisms that contribute to meat quality in swine.
Language
English
Location
UPLB Main Library Special Collections Section (USCS)
Call Number
LG 993.5 2014 A127 P66
Recommended Citation
Pontanal, Isabella Marie Padua, "The isolation and molecular characterization of Ghrelin gene in Philippine native pigs (Sus scrofa)" (2014). Undergraduate Theses. 2518.
https://www.ukdr.uplb.edu.ph/etd-undergrad/2518
Document Type
Thesis