Resistance screening of rice (Oryza sativa L.) to the sheath brown rot disease caused by pseudomonas fuscovaginae using pin-prick inoculation

Date

4-2014

Degree

Bachelor of Science in Agriculture

Major Course

Major in Plant Pathology

College

College of Agriculture and Food Science (CAFS)

Adviser/Committee Chair

Ireneo B. Pangga

Abstract

The research internship was conducted at the Plant Breeding, Genetics and Biotechnology Division, International Rice Research Institute, Los Baños, Laguna. It involved laboratory and greenhouse work that included culture media and inoculum preparation, inoculation, and disease assessment for resistance screening of rice to the sheath brown rot disease caused by Pseudomonas fuscovaginae. Disease resistance of 20 randomly selected accessions was evaluated by pin-prick inoculation to compare the virulence of two strains: IRRI 7007 and IRRI 6609. Disease severity was assessed at 14 days after inoculation in terms of the infected proportion of the inoculated sheath per plant. IRRI 7007 produced a significantly higher disease severity than IRRI 6609, which indicates that it is more virulent. Thus, IRRI 7007 was used to assess the resistance of 130 rice accessions in a second trial. A rating scale was devised based on the mean comparison results that were used to classify the accessions into resistant, intermediate and susceptible reactions. Cluster analysis followed the same classification and produced a more detailed rating scale. Out of the 130 accessions, 2 were highly susceptible, 7 were susceptible, 18 were moderately susceptible, 59 were moderately resistant and 44 were resistant based on cluster analysis.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

LG 993 2014 A3 /T34

Document Type

Thesis

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