In vitro efficacy of garlic (Allium sativum L.), oregano (Origanum vulgare L.), and turmeric (Curcuma longa L.) against the development of Ascaridia galli eggs from Philippine native chicken (Gallus gallus domesticus L.)

Date

4-2014

Degree

Bachelor of Science in Agriculture

Major Course

Major in Animal Science

College

College of Agriculture and Food Science (CAFS)

Adviser/Committee Chair

Veneranda A. Magpantay

Abstract

The efficacy of garlic, oregano, and turmeric in inhibiting larval development of Ascaridia galli eggs was evaluated in vitro. About 2000 to 2500 A. galli eggs were incubated per treatment which was replicated 5 times. Ethanolic extracts of the three plant materials were diluted in PBS to form a dose concentration of 50 mg/ml. Eggs in PBS was considered as the negative control whereas eggs in Albendazole (50 mg/ml) suspension acted as positive control. The samples were kept in room temperature and were examined on the 10th, 14th, and 21st day of incubation. Results revealed that the three plants were as effective as Albendazole in inhibiting the development of A.galli eggs on the 10th day of incubation. Garlic and turmeric were found to be effective compared with the negative control but were no longer as efficient as Albendazole on day 14 and day 21. Among the three plant extracts, garlic was the most effective in inhibiting the development of the eggs since it did not go beyond the morula stage until day 21. On the other hand, the number of eggs that formed into larval stage was lower in turmeric than in oregano. This study indicated that garlic, oregano and turmeric can be used to prevent A. galli infestation but supplementation must be programed in order to retain their efficacy.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

LG 993.5 2014 A3 /V55

Document Type

Thesis

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