Test for effectivity and identification of bacteria as biological control agents against sheath blight of corn

Date

12-2012

Degree

Bachelor of Science in Agriculture

Major Course

Major in Plant Pathology

Adviser/Committee Chair

Marina P. Natural

Co-adviser

Jocelyn T. Zarate

Restrictions

Restricted: Not available to the general public. Access is available only after consultation with author/thesis adviser and only to those bound by the confidentiality agreement.

Abstract

Bacteria isolated from different soil samples were preliminarily screened for potential antagonistic effect against Rhizoctonia solani using the multiple cultivation technique. A total of nineteen isolates showed growth inhibitory effect towards the sheath blight pathogen and were subjected to a final screening using the dual culture technique. Only three isolates (DPV 9, DPV 31, DPV 33) exhibited consistency in inhibiting the growth of the fungal pathogen as observed by the inhibition zone produce. Microscopic examination revealed the collapsed and swollen hyphae of the fungus in the presence of the bacterial antagonists. The results of the characterization of the isolates based on cultural, morphological, physiological, and biochemical test suggest that the bacteria belong in the genus Bacillus and most probably Bacillus licheniformis. Greenhouse evaluation of their potential biological control ability revealed that the three bacterial isolates cannot eradicate the sheath blight pathogen as the lesion produced in the treated plants are not significantly different from the untreated plants. However, two of the isolates screened namely, DPV 9 and DPV 33 showed potential biological control ability in suppressing R. solani if applied as protectant.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

LG 993.5 2012 A3 /V45

Document Type

Thesis

This document is currently not available here.

Share

COinS