Diversity analysis of sweet potato (Ipomoea batatas (L.) Lam.) germplasm collections of the National Plant Genetic Resources Laboratory (NPGRL) using microsatellite markers

Date

10-2012

Degree

Bachelor of Science in Agriculture

Major Course

Major in Agronomy

College

College of Agriculture and Food Science (CAFS)

Adviser/Committee Chair

Antonio G. Lalusin

Abstract

Abstract: Microsatellite markers or simple sequence repeat (SSR) were used to determine the genetic diversity of sixty four (64) sweetpotato accessions of National Plant Genetic Resources Laboratory, Institute of Plant Breeding in the University of the Philippines, Los Baños. Eight out of the ten SSR primer pairs, which are based on three different crops were tested and amplified with a range of 2 to 8 polymorphic fragments per primer. Resulting unique banding patterns for the 10 SSR markers ranged from 1 to 21. The resolving power of the SSR markers used, measured as polymorphism information content (PIC), ranged from 41.14% to 83.90%, which indicates high genetic diversity. Cluster analysis using Jaccard?s coefficient at 0.51 showed high genetic diversity. Dendrogram generated five clusters. Grouping based on geographic location was observed.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

LG 993.5 2012 A3 /R36

Document Type

Thesis

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