Substrate optimization of biosurfactant production by Saccharomyces cerevisiae 2031 using molasses and coco paring meal by response surface methodology.

Date

4-2013

Degree

Bachelor of Science in Chemical Engineering

College

College of Engineering and Agro-Industrial Technology (CEAT)

Restrictions

Restricted: Not available to the general public. Access is available only after consultation with author/thesis adviser and only to those bound by the confidentiality agreement.

Abstract

In this study, substrate optimization of biosurfactant production was done using molasses as additional carbon source to glucose and coco paring meal as nitrogen source. A 3FI design was first used for pre-optimization stage. The optimum condition utilized by Box-Behnken design with 5 center points was based on three parameters namely molasses concentration, coco paring meal concentration and pH. Optimum conditions were determined as pH 5.69, 10.60% (w/v) molasses and 7.27%(v/v) coco paring meal. At these conditions, responses biomass concentration and emulsification index obtained were 6.43g/L and 82.81, respectively. Effect Of fermentation time on the two responses using shake-flask method and bioreactor system was also studied. With the first method , the maximum biomass concentration was obtained after 3 days of shaking (5.55± 0.05g/mL) while the highest emulsification activity was attained after 4 days of shaking (84.60±0.25). On the other hand, the bioreactor system's maximum yield for both biomass (6.25±0.05g/L) and emulsification activity (93.33) was observed after 4 days of shaking. Analyses of the total sugar, protein content and surface tension reduction of biosurfactant were also done.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

LG 993.5 2013 E62 M35

Document Type

Thesis

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