Optimization of dilute sulfuric acid pretreatment of rice straw for ethanol production

Date

4-2010

Degree

Bachelor of Science in Chemical Engineering

College

College of Engineering and Agro-Industrial Technology (CEAT)

Adviser/Committee Chair

Rex B. Demafelis

Committee Member

Fidel Rey P. Nayve

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Abstract

Lignocellulosic biomass used in ethanol production is initially pretreated to make the cellulose in the biomass accessible to hydrolyzing agents like enzymes. This involves the optimization of the dilute sulfuric acid pretreatment of rice straw for ethanol production. Ethanol fermentation of the acid hydrolysate after optimal pretreatment of the rice straw was done using the Saccharomyces cerevisiae 2030. Using the Box-Behnken design of experiment, the optimized pretreatment parameters were autoclave temperature, H2SO4 concentration and reaction time while the response variables were reducing sugar and total sugar yields. It was found that the optimum pretreatment condition based on the reducing sugar yield were 114.86 oC, 0.16 M H2SO4, and 41.34 min. At these optimum conditions, the reducing sugar concentrations in the hydrolysate were 14.125 mg/mL and 20.262 mg/mL using Acellarase 1500 and Novozymes’ enzymes, respectively; the corresponding ethanol levels were 0.58 % (v/v) and 0.90 % (v/v).

Language

English

Location

UPLB College of Engineering and Agro-Industrial Technology

Document Type

Thesis

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