Genetic integrity of selected Oryza sativa L. variety detected by Simple Sequence Repeat (SSR) analysis

Date

4-2010

Degree

Bachelor of Science in Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Merlyn S. Mendioro

Co-adviser

Kenneth M. Mcnally

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Abstract

Genetic integrity of 36 accessions of Oryza sativa L. was determined. More than 400 accessions of Oryza were sent to USDA from IRRI in different times for the past four decades. After verifications from IRRI, at least 36 accessions were found to have discrepancies in the seed files. Simple Sequence Repeat (SSR) analysis using 16 primers was done to compare each accession. A radial tree was generated and the similarity of each accession was computed. Out of the 36 accessions, only four were evaluated to retain their genetic integrity which are the Asse y Pung, Dam, Tsipala 421, and Vary Vato accessions which have similarities of 0.63, 0.65, 0.67, and 0.79, respectively. The divergence of the accessions can be attributed to three possible reasons. First, it can be due to the plant’s adaptation to a different environment resulting to genotypic and phenotypic changes. Second, it can be due to the series of seed regeneration that the accessions have undergone. Inaccurate regenerations may result to variations in the accessions. Lastly, it can be due to mistakes in file keeping. Thousands of germplasms are being kept and conserved making it hard to file each of the individual accessions.

Language

English

Location

UPLB Main Library Special Collections Section

Document Type

Thesis

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