Determination of lead in blood by Differential Pulse Anodic Stripping Voltammetry (DPASV).

Date

5-2012

Degree

Bachelor of Science in Chemistry

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Reyes, Charisse T.

Committee Member

Rodriguez, Evelyn B.

Abstract

This study developed a working method for the analysis of lead (II) in blood using two electrochemical techniques namely Cyclic Voltammetry (CV) and Differential Pulse Anodic Stripping Voltammetry (DPASV). Cyclic Voltammetry was employed to determine the electrochemical behavior of lead (II) with the hanging mercury drop electrode (HMDE) and to identify the potential redox range of lead (II). It was found to detect lead (II) in solution at ppm levels at the range of -320 to -375 mV with respect to the Ag/AgCl reference electrode. Optimized experimental parameters for DPASV were determined to be: 0.1 M HCl solution as the supporting electrolyte; deposition time of 300.0 seconds; and deposition potential of -1000 mV. Linear responses were obtained in two different concentration ranges (10 ppb to 100 ppb and 0.1 ppm to 1 ppm). The DPASV technique was found to be highly sensitive in the analysis of lead at low concentrations, having a sensitivity of 11.36 μA/ppm. A limit of detection and limit of quantification of 1.92 ppb and 6.41 ppb, respectively, were obtained. The blood lead levels of smoking BS Chemistry students (0.24 ppm to 0.36 ppm) were found to be higher than those of non-smoking students (0.08 ppm to 0.13 ppm). Local cigarette samples were also analyzed by DPASV to determine its potential factor as the source of lead toxicity in blood. It was found out that the lead concentration in cigarettes ranges from 0.13 ppm to 2.31 ppm.

Language

English

Location

UPLB Main Library Special Collections Section

Call Number

LG 993.5 2012 C42 A38

Document Type

Thesis

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