Production of single-cell protein (SCP) from yeast Candida sp grown in cassava starch hydrolyzates.

Date

2012

Degree

Bachelor of Science in Chemical Engineering

College

College of Engineering and Agro-Industrial Technology (CEAT)

Restrictions

Restricted: Not available to the general public. Access is available only after consultation with author/thesis adviser and only to those bound by the confidentiality agreement.

Abstract

Single Cell Protein (SCP) production from cassava starch hydrolyzate was done using a promising strain of the yeast Candida utilis 2164 of the National Institute of Molecular Biology and Biotechnology (BIOTECH). Initially, flask screening experiment was made to select the yeast strain (Candida sp) and initial sugar concentration to be used in the bioreactor experiments. Based on increase on biomass concentration after 24 hours of fermentation, Candida utilis 2164(7% initial sugar concentration) was selected for the bioreactor experiments. Different aeration/agitation conditions showed that Candida utilis 2164 could produce the SCP biomass from 6.28 g/L to 7.9g/L. Generally, higher aeration and agitation gave higher biomass concentrations. The oxygen mass transfer coefficient (kLa) was also determined (Dynamic method) to examine the effect of aeration and agitation (as single"lumped" parameter) on the performance of the selected yeast strain, and for further scale-up of the process. The highest kLa a attained was 0.007s⁻¹ (2vvm/450rpm). The results indicate that SCP for animal feed can be produced from cassava starch hydrolyzate using the selected yeast Candida utilis 2164 with initial sugar concentration of 7%. Aeration and agitation had some positive effect on SCP production, and the best combination was at 2vvm aeration and 450rpm agitation.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

LG 993.5 2012 E62 O26

Document Type

Thesis

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