Application of repeated batch fermentation process with cell recycle for the molasses-based ethanol fermentation of a promising local biotech yeast strain

Date

4-2012

Degree

Bachelor of Science in Chemical Engineering

Major Course

Major in Microbiology

College

College of Engineering and Agro-Industrial Technology (CEAT)

Restrictions

Restricted: Not available to the general public. Access is available only after consultation with author/thesis adviser and only to those bound by the confidentiality agreement.

Abstract

Repeated batch culture with cell recycle was applied for the ethanol fermentation from molasses using a promising yeast strain from the National Institute of Molecular Biology and Biotechnology, in order to compare the performance of this system with a conventional (replicated, with non-cell-recycle) batch system. The promising yeast strain, Saccharomyces cerevisiae 2013, was selected via flask screening experiment, based on the final ethanol concentration, productivity and fermentation efficiency. Single batch fermentation experiment showed that the strain's performance was at par with the strain used in the industry. Repeated batch fermentation for 5 cycles showed that the fermentation performance improved with an increase in cycle number. However, the performance was observed to level-off at an optimum cycle number with a yield coefficient of 0.52g ethanol per g sugar., a productivity of 6.75 g ethanol/l-h and a fermentation efficiency of 96.6%. The cell-recycle repeated batch culture was better by factors of 3 productivity and 1.08 for efficiency, compared to a single conventional batch culture replicated 5 times without cell recycle. These results indicate that this system can be an alternative option for the improvement of ethanol production in local distilleries.

Language

English

Location

UPLB College of Engineering and Agro-Industrial Technology (CEAT)

Call Number

LG 993.5 2012 E62 G87

Document Type

Thesis

This document is currently not available here.

Share

COinS