Microbial production of hydrogen gas from Jatropha curcas seed cake

Date

4-2009

Degree

Bachelor of Science in Chemical Engineering

College

College of Engineering and Agro-Industrial Technology (CEAT)

Adviser/Committee Chair

Jovita L. Movillon

Co-adviser

Chay Bihn Pham

Committee Member

Butch G. Bataller, Laura J. Pham

Abstract

The production of hydrogen using Jatropha curcas seed cake as a fermentation substrate by selected microorganisms was carried out in a batch process by a laboratory scale. Ten bacterial species were screened for gas production using batch fermentation in a 250-mL medium containing 30g/L Jatropha curcas seed cake with an initial ph of 6.5 at 30-37°C. The preliminary microbial screening shows that Enterobacter aerogenes 1764 evolved the highest amount of gas at 602.5mL/L medium. Parametric and optimization studies were performed in order to determine the effects of Jatropha curcas Seed Cake concentration (20, 30, 40 g/L medium), temperature (30, 37, 45°C), and initial (5.5, 6.5, 7.5) on the microbial hydrogen production by Enterobacter aerogenes 1764. Twelve randomly selected parameter combinations were performed and results of hydrogen evolution (mL/L medium), cell biomass concentration (g/L), total sugar concentration (g/L) and final pH were obtained. Using Response Surface Analysis regression models for the four responses were obtained . Quadratic models were chosen in order to predict the effects of the three parameters on the responses. the highest volume of hydrogen produced was at 30g/L followed by 30g/L. Hydrogen evolution is highest at pH 7.5, followed by 6.5 and then 5.5. Moreover, the total sugar residue is highest at pH 6.5 and then 7.5, and lastly 5.5.On the average, hydrogen evolution is highest at 37°C followed by 30°C and 45°C while the residual sugar concentration is highest at 37°C followed by 45°C and 30°C . Using the numerical optimization method, the desirable treatment combination that will produce the maximum amount of hydrogen is 26.97g/L Jatropha curcas seed cake concentration, 39.66°C, initial pH5.5 producing 3.0691mL hydrogen/L medium with 83.4% desirability.

Language

English

LC Subject

Jatropha, Fossil fuels--Toxicology

Location

UPLB College of Engineering and Agro-Industrial Technology

Call Number

LG 993.5 2009 E62 G48

Document Type

Thesis

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