Mycelial growth of Pleurotus eryngii (DC.: Fr.) Quel as affected by culture media, darkness, light conditions, and temperatures

Date

4-2014

Degree

Bachelor of Science in Agriculture

Major Course

Major in Plant Pathology

College

College of Agriculture and Food Science (CAFS)

Adviser/Committee Chair

Teresita U. Dalisay

Restrictions

Restricted: Not available to the general public. Access is available only after consultation with author/thesis adviser and only to those bound by the confidentiality agreement.

Abstract

Pleurotus eryngii (DC.:Fr.) Quel was grown on different culture media and subjected to darkness, light conditions, and temperatures to determine the effect of different factors on its mycelial growth. During eleven days of incubation and daily measurement, Potato White sugar ?Gulaman‟ bars Agar (PSGA) was found out to be the suitable medium for starter culture of P. eryngii among the three agar media screened. This is due to the higher colony growth (79.97 mm) and faster growth rate (6.54 mm/day) obtained in PSGA, as well as very abundant mycelial density. Having known of the suitable medium, cultures of P. eryngii on plated PSGA medium were subjected to Continuous Light(CL), Continuous Dark (CD), and Alternate Light and Dark (ALD), and temperature treatments of 30°, 27°, and 24°C. Colony growth and growth rate obtained for all treatments have values close to each other, thus, statistical analysis of the data gathered yielded P-values which are not significant. Also, mycelial density of P. eryngii did not vary among treatments. These results signified that subjecting P. eryngii grown on PSGA medium to different light conditions and temperatures, have no effect on its mycelial growth. For spawn preparation, bottled sorghum seeds were used as culture media of P.eryngii and incubated to the same darkness, light conditions and temperatures as that of agar medium. Absence of light and temperature has a significant effect on the mycelial growth of P. eryngii on bottled sorghum seeds. Continuous Dark and 27°C individually favored faster spawn run (106.15 mm and 108.35 mm) and growth rate (7.33 mm/day and 7.44 mmm/day) of P. eryngii after sixteen days of incubation. Furthermore, P. eryngii grown on standard substrate was only subjected to Continuous Dark condition on a normal room temperature, and it took only fourteen days to reach an average spawn run of 77.15 mm.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

LG 993.5 2014 A3 /G37

Document Type

Thesis

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