Research internship on corn DNA profiling and Plant Variety Protection (PVP) application in the Philippines

Date

12-2014

Degree

Bachelor of Science in Agricultural Biotechnology

College

College of Agriculture and Food Science (CAFS)

Adviser/Committee Chair

Hayde F. Galvez

Abstract

The research internship was conducted at the Crop Science Cluster ? Institute of Plant Breeding, College of Agriculture, University of the Philippines Los Banos from March 2014 to October 2014. The field activities as well as documentation were conducted at the National Seed Foundation (NSF) unit and the Genetics Laboratory. While the second part of the internship, which was on molecular analysis was conducted at the Genetics Laboratory. The research internship aims to make the intern undergo and learn the requirements to file a Plant Variety Protection (PVP) in the Philippines, specifically corn. The intern reviewed relevant documents and literatures pertaining to PVP in international context and in the Philippines. Distinctness, Uniformity, and Stability Test (DUS test) was performed to examine the candidate varieties being applied, as indicated in the Corn PVP application test guidelines. Out of 10 entries planted, only seven (7) germinated and successfully grew into mature plants. Forty-six (46) characters, consisting of 30 qualitative and 16 quantitative traits were evaluated in comparison with the standard traits of corn as catalogued by Philippine Plant Variety Protection Office. A sample DNA profile characterization among corn entries was also generated and analyzed by the intern as additional data to support the PVP application. Microsatellite markers previously mapped in corn was used in the molecular analysis. The DNA profile reveals genetic variations among the candidate corn varieties and control entries, thus providing additional evidence for being a distinct variety. The geometric distance among the candidate corn entries was analyzed using the NTSYSpc v.2.1 software (Rohlf, 2000), and four clusters of genetic differentiation were revealed at simple matching coefficient of ca. 0.80. Incorporating the morphological (field) characterization of the candidate corn varieties grown under two distinct seasons, and with more DNA molecular data, the Institute of Plant Breeding is pursuing the PVP application following the guidelines and requirements set by the Philippine PVP for corn.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

LG 993 2014 A127 /U58

Document Type

Thesis

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