Parametric study on ethanol fermentation of sweet sorghum [Sorghum bicolor (L.) Moench 'SPV 422"] syrup using biotech yeast strains under sterilized and unsterilized conditions

Date

7-2015

Degree

Bachelor of Science in Chemical Engineering

College

College of Engineering and Agro-Industrial Technology (CEAT)

Adviser/Committee Chair

Rex B. Demafelis

Restrictions

Restricted: Not available to the general public. Access is available only after consultation with author/thesis adviser and only to those bound by the confidentiality agreement.

Abstract

Ethanol fermentation was conducted using sweet sorghum (SPV 422) syrup as substrate to evaluate the effects of yeast strain, pH, inoculum loading and sterilization on the ethanol concentration and sugar consumption. Two local BIOTECH yeast strainswereused namely: Saccharomyces cerevisiae 2011 and Saccharomyces cerevisiae 2055. Among the parameters tested pH, inoculum loading and sterilzation were found to have a significant effect on the final eyhanol concentration. Yeast strain and sterilization had a significant effect on reducing sugar consumption, while inoculum loading had a significant effect on total sugar consumption. Saccharomyces cerevisiae 2011 was selected over Saccharomyces cerevisiae 2055 since higher ethanol concentration and sugar consumption wrre achieved using this strain. The highest ethanol concentration and sugar consumption were achieved using this strain. The highest ethanol concentration obtained was 8.73% with 94.69% reducing sugar consumption. A fermentation profile using the optimum conditions (pH 4.5, 10%inoculum loading and with sterilization) was constructed and a fermentation efficiency of 84.66% was achieved.

Language

English

Location

UPLB College of Engineering and Agro-Industrial Technology (CEAT)

Call Number

LG 993.5 2015 E62 /R45

Document Type

Thesis

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