Date

3-2009

Degree

Bachelor of Science in Chemical Engineering

College

College of Engineering and Agro-Industrial Technology (CEAT)

Adviser/Committee Chair

Rex B. Demafelis

Co-adviser

June Owen O. Nacorda

Committee Member

Amelia E. Parao, Catalino G. Alfafara

Abstract

The microalga Navicula sp was cultivated using photo bioreactors and its oil was solvent-extracted using hexane as solvent. The oil that was obtained was then subjected to lipid analysis. The media that was used for the cultivation is Modified BG-11. The effect of algal mass to solvent volume ratio such as 1:4, 1:5, 1:6 on the oil yield was determined. A mean oil yield of 3.55%, 7.86% and 9.73% was achieved for 1:4, 1:5, 1:6 ratios, respectively. Statistically, the treatments are not significantly different but the 1:6 ratio was chosen to be the most favorable ratio since it has the highest oil yield achieved and due to the errors that was committed in 1:4 and 1:5 ratio. The variation of soaking time (12, 24 and 36 hours) was also performed to determine its effect on the oil yield. The corresponding percent oil yield for 12, 24 and 36 hours were 1.40%, 9.46%, 10.17% respectively. It was observed that as the soaking time increases the oil yield also increases but up to a certain point only. The most favorable soaking time is 24 hours with 9.46% oil yield. Thin layer chromatography was used to determine the components of the extracted oil. The lipid composition of Navicula sp is 84.38% triacylglycerols, and 15.62% Sterol.

Language

English

LC Subject

Microbiology, Biomass energy.

Call Number

LG 993 2009 E62 D43

Document Type

Thesis

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