Optimization study on the fermentation of alginic acid and mannitol from macroalgae (Sargassum spp.) using Saccharomyces cerevisiae 2055 for bioethanol production

Date

6-2015

Degree

Bachelor of Science in Chemical Engineering

College

College of Engineering and Agro-Industrial Technology (CEAT)

Adviser/Committee Chair

Rex B. Demafelis

Restrictions

Restricted: Not available to the general public. Access is available only after consultation with author/thesis adviser and only to those bound by the confidentiality agreement.

Abstract

Optimization of fermentation condition was developed to improve conversion of alginic acid and mannitol to bioethanol. A central composite design (CCD) was used to generate a quadratic model that described the effect of temperature, pH, and substrate ratio on the ethanol production and percent sugar production. Generally, all factors were found to have significant effect on ethanol production while temperature is the only significant factor affecting reducing sugar consumption. The results were analyzed using response surface methodology (RSM), and the optimum fermentation conditions for temperature, pH, and substrate ratio were 30°C, 4.01, and 0.51: 0.49 (sugar from alginic acid: mannitol)' respectively. The highest ethanol produced performing optimum conditions was 2.8282 mg/mL which has 1.0611% difference from the predicted maximum ethanol concentration. Sole fermentation of alginic acid and mannitol was also performed having ethanol concentrations f 2.74 mg/mL and 2.30 mg/mL, respectively. This showed that fermentation of alginic acid is highly favored compared to mannitol in the substrate mixture.

Language

English

Location

UPLB College of Engineering and Agro-Industrial Technology (CEAT)

Call Number

LG 993.5 2015 E62 /L39

Document Type

Thesis

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