Optimization study on the fermentation of cellulose and mannitol from macroalgae (Sargassum spp.) using Saccharomyces cerevisiae 2055 for bioethanol production

Date

6-2015

Degree

Bachelor of Science in Chemical Engineering

College

College of Engineering and Agro-Industrial Technology (CEAT)

Adviser/Committee Chair

Rex B. Demafelis

Restrictions

Restricted: Not available to the general public. Access is available only after consultation with author/thesis adviser and only to those bound by the confidentiality agreement.

Abstract

The optimum temperature, pH and sugar ratio for the fermentation of cellulose and mannitol extracted from Sargassum spp. were determined. Saccharomyces cerevisiae 2055 was used in the conversion of sugars into ethanol. Based on Response Surface Methodology (RSM Central Composite Design (CCD) analysis, a quadratic model fit is significant and 19 optimum conditions were obtained. Among the optimum points, a set with 30°C, pH 5.0 and cellulose to mannitol ratio of 3:1 with an ethanol concentration of 4.154 mg/mL was chosen. Crude extracted mannitol was also compared to commercial mannitol in terms of ethanol concentration and fermentation efficiency. Final ethanol concentration of 4.077±0.133 mg/mL and fermentation efficiency of 72.495±6.827% were obtained from cellulose with crude mannitol extract medium while final ethanol concentration of 4.096±0.385 and fermentation efficiency of 72.585±6.445% for cellulose with commercial mannitol medium.

Language

English

Location

UPLB College of Engineering and Agro-Industrial Technology (CEAT)

Call Number

LG 993.5 2015 E62 /G66

Document Type

Thesis

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