Optimization study on the fermentation of cellulose and mannitol from macroalgae (Sargassum spp.) using Saccharomyces cerevisiae 2055 for bioethanol production
Date
6-2015
Degree
Bachelor of Science in Chemical Engineering
College
College of Engineering and Agro-Industrial Technology (CEAT)
Adviser/Committee Chair
Rex B. Demafelis
Restrictions
Restricted: Not available to the general public. Access is available only after consultation with author/thesis adviser and only to those bound by the confidentiality agreement.
Abstract
The optimum temperature, pH and sugar ratio for the fermentation of cellulose and mannitol extracted from Sargassum spp. were determined. Saccharomyces cerevisiae 2055 was used in the conversion of sugars into ethanol. Based on Response Surface Methodology (RSM Central Composite Design (CCD) analysis, a quadratic model fit is significant and 19 optimum conditions were obtained. Among the optimum points, a set with 30°C, pH 5.0 and cellulose to mannitol ratio of 3:1 with an ethanol concentration of 4.154 mg/mL was chosen. Crude extracted mannitol was also compared to commercial mannitol in terms of ethanol concentration and fermentation efficiency. Final ethanol concentration of 4.077±0.133 mg/mL and fermentation efficiency of 72.495±6.827% were obtained from cellulose with crude mannitol extract medium while final ethanol concentration of 4.096±0.385 and fermentation efficiency of 72.585±6.445% for cellulose with commercial mannitol medium.
Language
English
Location
UPLB College of Engineering and Agro-Industrial Technology (CEAT)
Call Number
LG 993.5 2015 E62 /G66
Recommended Citation
Gomez, Czaneil C., "Optimization study on the fermentation of cellulose and mannitol from macroalgae (Sargassum spp.) using Saccharomyces cerevisiae 2055 for bioethanol production" (2015). Undergraduate Theses. 4016.
https://www.ukdr.uplb.edu.ph/etd-undergrad/4016
Document Type
Thesis