Titer determination of Mycoplasma gallisepticum specific immune sera produced in chickens (Gallus gallus domesticus) and rabbits (Oryctolagus cuniculus) using MG 6/85 propagated antigen in the hemagglutination inhibition test

Date

6-2015

Degree

Doctor of Veterinary Medicine

College

College of Veterinary Medicine (CVM)

Adviser/Committee Chair

Joseph P. Olarve

Abstract

Chronic Respiratory Disease (CRD), a disease caused by Mycoplasma gallisepticum (MG) is of economic importance to the poultry industry worldwide. Hemagglutination inhibition (HI) Test is performed for disease diagnosis and detection of immune response to vaccination. Such test is not regularly performed because of the lack of test reagents such as antigen and control sera. This study aimed to develop immune sera specific for MG in both chickens and rabbits, and to prepare antigen to be used for the HI test. Initial immunization in chickens using a commercial killed bacterin resulted to a rise in mean titers up to 1:144 in the last bleed, 28 days after the last booster. HI determination used an imported MG antigen. Live vaccine strains of MG were propagated in Frey?s medium to make antigen for the HI test while immunogens were produced by inactivating the propagated MG in 2% formalin mixed with incomplete and complete Freund?s adjuvant. Titers in rabbits were monitored using the HI test but the highest titersonly reached up to 1:20. Further studies using alternative vaccines and vaccination protocols are recommended. This is the first report on the development of immune sera and propagation of MG antigen to be used as diagnostic reagents in anMG diagnostic test for Philippine poultry.

Language

English

Location

UPLB College of Veterinary Medicine (CVM)

Call Number

LG 993.5 2015 V4 S55

Document Type

Thesis

This document is currently not available here.

Share

COinS