Date

3-2009

Degree

Bachelor of Science in Chemical Engineering

College

College of Engineering and Agro-Industrial Technology (CEAT)

Adviser/Committee Chair

Rex B. Demafelis

Co-adviser

Francis J. Del Rosario, Fidel Rey P. Nayve, Jr.

Committee Member

Francisco B. Elegado, Jovita L. Movillon

Abstract

Ethanol production from agricultural waste such as sugarcane bagasse provides environmental, economic, and energy security benefits. However, current costs of the technology are too high for use of cellulosic ethanol as fuel, with the pretreatment and cellulose hydrolysis presenting high priority for cost reduction.Furthermore, developing the fermentation process would increase the biomass conversion, ethanol concentrations and productivity to reduce the cost. In this study, optimization was done on dilute acid pretreatment in an autoclave and enzymatic saccharification of sugarcane bagasse (Saccharum officinarum) prior to ethanol fermentation using Saccharomyces cerevisiae and Pachysolen tannophilus. The highest yield of total sugar was obtained with pretreatment conditions of 20 psig, 30 minutes and 0.4% (v/v) sulfuric acid upon constant enzyme loading. Optimal enzyme loading using Accelerase 1000(Genencor Intl.) was 45.98 FPU/mL for 9% (w/v) pretreated sugarcane bagasse using the optimum conditions for ACCELERASE. Simultaneous Saccharification and Fermentation (SSF) or separate Hydrolysis and Fermentation (SHF) were tested using single or mixed cultures of P.tannophilus ans S.cerevisiae. SSF using mixed cultures of improved ethanol yield by 6.11% while SHF using mixed cultures overcame glucose inhibition for P. tannophilus which further improved fermentation by 7.29%, based on S.cerevisiae fermentation of pre-treated bagasse. In this stuidy, 0.167mg ethanol per mg bagasse was obtained which corresponds to 0.278mg ethanol per mg cellulose and hemicellulose.

Language

English

LC Subject

Chemical engineering

Call Number

LG 993.5 2009 E62 O25

Document Type

Thesis

Download

Share

COinS