Prevalence of F18 and STb gene and the antimicrobial susceptibility profile of enterotoxigenic Escherichia coli from piglets with post-weaning diarrhea in selected provinces in Luzon

Date

6-2015

Degree

Doctor of Veterinary Medicine

College

College of Veterinary Medicine (CVM)

Abstract

To determine the prevalence of enterotoxigenic Escherichia colipathotypes among post-weaning diarrheic piglets, 111 isolates were tested for the presence of both F18 and STbgenes using polymerase chain reaction. Results showed that 36.04% and 63.96% of the isolates were positive for F18 adhesin gene and STb gene, respectively. Combined with previous data on beta-hemolysis, chosen representatives were tested against 9 antimicrobial drugs using broth microdilution technique for the determination of the minimum inhibitory concentration (MIC). Over 80% of the isolates were found to be multi-drug resistant with 100% showing resistance to gentamicin. This study has shown the presence of both F18 and STb genes in piglets with post-weaning diarrhea with a high prevalence rate. These results represent one of the first characterizations of E. coli pathotypes in post-weaning diarrheic piglets in the Philippines.

Language

English

Location

UPLB College of Veterinary Medicine (CVM)

Call Number

LG 993.5 2015 V4 A58

Document Type

Thesis

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