Preliminary evaluation of Direct Immunohistochemical Test (DRIT) of hippocampal touch impression smears using a monospecific polyclonal antibody for rabies virus detection

Date

4-2013

Degree

Doctor of Veterinary Medicine

College

College of Veterinary Medicine (CVM)

Restrictions

Restricted: Not available to the general public. Access is available only after consultation with author/thesis adviser and only to those bound by the confidentiality agreement.

Abstract

Rabies is an important zoonotic disease that needs to be eradicated worldwide. Accurate and rapid postmortem detection testing for the rabies virus in regional laboratories is needed. Thus, evaluation of DRIT of hippocampal touch impressions of suspected rabid dogs using the monospecific N protein polyclonal antibody developed by RITM was done. One hundred sixty acetone-fixed hippocampal touch impressions were subjected to DRIT. One hundred thirteen (70.6%) out of 160 samples tested positive for rabies viral antigen (RVA) and 47 (29.4 %) out of 160 samples tested negative for RVA. No false positive and false negative results were obtained. The same results were obtained using dFAT. DRIT was able to detect low to high concentrations of RVA in the hippocampal touch impressions based on the grading distribution. The 100% sensitivity, specificity and over-all accuracy of DRIT using monospecific polyclonal antibodies was comparably similar with dFAT. In conclusion, DRIT using monospecific N protein polyclonal antibody can be used as an alternative post-mortem diagnostic test for rabies virus in suspected rabid dogs.

Language

English

Location

UPLB College of Veterinary Medicine (CVM)

Call Number

Thesis

Document Type

Thesis

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