Parametric study on the growth and ethanol production of amylase-producing yeast isolate BIOTECH Rttwy-2 using Taro (VG-9) flour as substrate

Date

6-2016

Degree

Bachelor of Science in Chemical Engineering

College

College of Engineering and Agro-Industrial Technology (CEAT)

Adviser/Committee Chair

Marilyn C. Del Barrio

Restrictions

Restricted: Not available to the general public. Access is available only after consultation with author/thesis adviser and only to those bound by the confidentiality agreement.

Abstract

The study evaluated the effects of temperature, amount of inoculum, and Taro (VG-9) flour concentration on the growth and ethanol production of BIOTECH (Rttwy-2, an amylolytic and ethanol-producing yeast isolated from fermenting taro. Results of the two-level factorial experiment showed that all of the three factors significantly affected the growth and ethanol production of Rttwy-2. However, all their interactions were found to be insignificant. Temperature and taro flour concentration positively affected both growth and ethanol production, while amount of inoculum negatively affected both. Highest biomass (4,16 x 10^8 cells/mL) and ethanol concentration (4.97 % v/v) were produced at 40 °C, 3% inoculum and taro flour concentration of 40 g/L. On the other hand, the lowest biomass (2.32 x 10^8 cells/mL and ethanol concentration (3.078 % v/v) occurred at 30°C, 10% inoculum, and taro flour concentration of 30 g/L. Based on ethanol production, BIOTECH Rttwy-22 is a good fermentation microorganism since it can solely convert starch to ethanol. However, if used as a component of a starter culture, it may inhibit the growth of other microorganisms at higher cell concentration. Therefore it can be used as a sole starch-to-ethanol fermentation microorganisms, but further studies are still needed to determine if BIOTECH Rttwy-2 can be used as a component of a taro-rice wine starter culture.

Language

English

Location

UPLB College of Engineering and Agro-Industrial Technology (CEAT)

Call Number

LG 993.5 2016 E62 /R47

Document Type

Thesis

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