Assessing the salinity tolerance of selected rice (Oryza sativa L.) varieties at the seedling and vegetative growth stages and genetic characterization using single nucleotide polymorphisms.

Date

10-2012

Degree

Bachelor of Science in Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Villa, Neilyn O.

Co-adviser

Gregorio, Glenn B

Abstract

The salinity tolerance at seedling and vegetative stages of growth in 12 rice (Oryza sativa L.) varieties were evaluated by measuring morphological parameters and yield components, and single nucleotide polymorphism (SNP) genotyping. It was observed that untreated plants (control) had shorter shoot lengths (30.7 to 111.8 cm) than treatments for salinity at the seedling stage (42.0 to 112.5 cm) and at the vegetative stage (59.5 to 112.5 cm). Plants salinized at both the seedling and vegetative stages had a shorter shoot length (42.0 to 105.5 cm) compared to other treatments. Genotypes salinized at both seedling and vegetative stages had the longest average days to flowering (74.33 days) than the control (64.17 days). Plants salinized for the seedling stage (SNN) and the vegetative stage had average days to flowering of 70.58 and 73.75, respectively. Relatively, the percent reduction in shoot biomass fresh weight was observed to be significantly lower among genotypes treated with salinity. Lower percent reduction in plant height, shoot fresh weight, number of filled and unfilled grains and 100-grainweight are associated with greater tolerance to salinity. Analysis of variance showed significant interactions between salinity and variety for reduction in number of filled and unfilled grains and reduction in 100 grain weight. No specific genotype was evaluated as tolerant at both seedling and vegetative stages. Molecular genotyping using 384 SNP markers resulted in a dendogram in which there was no consistent clustering of tolerant varieties at the seedling stage, vegetative stage, and both stages.

Language

English

Location

UPLB Main Library Special Collections Section

Call Number

Thesis

Document Type

Thesis

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