Identification of novel Quantitative Trait Loci (QTLs)for submergence tolerance in Oryza sativa L. derived from moderately tolerant IR64 variety

Date

4-2013

Degree

Bachelor of Science in Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Dela Viña, Celia B.

Abstract

To search for novel quantitative trait loci (QTLs), phenotyping was done by conducting submergence screening of 540 families of the F7 recombinant inbred line (RIL) population which was developed in the International Rice Research Institute (IRRI) from the cross between the submergence susceptible IR42 and moderately tolerant variety IR64 population. A subset of 94 families representing the two extreme phenotypic tails was used for genotyping and QTL analysis. Phenotype data showed transgressive segregation which indicated several families having a higher survival rate than the donor parent IR64 and lower survival rate than IR42. Using six polymorphic SSR markers and 52 polymorhic SNP markers, two potential novel QTLs were identified from the largest QTL was on chromosome 6 with a LOD score of 3.79 and additive effect of 17.45. The identification of three non-SUB1 QTLs from IR64 suggests that this variety may have potential QTLs or genes that are independent of the SUB1A gene and can possibly be combined with SUB1 QTL through marker-assisted backcrossing to increase submergence tolerance in rice varieties that are planned in flood-prone areas.

Language

English

Location

UPLB Main Library Special Collections Section

Call Number

Thesis

Document Type

Thesis

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