Toxicological test of Fungal- based organic colorant using chicken embryo assay, disk- diffusion assay, and micronucleus test.

Date

3-2013

Degree

Bachelor of Science in Biology

Major Course

Major in Microbiology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Tambalo, Fides Z.

Abstract

Toxicology of colorant produced from Monascus purpureus M1018 was done through chicken embryo assay, disk-diffusion assay, and micronucleus test. Water-based colorant and oil based colorant were tested for the presence of toxicity using chicken embryo assay. The positive control was phenol, a substitute for citrinin, and the negative controls were sterilized distilled water and sterilized oil. Results showed 100% embryo viability after force hatching of the eggs from all the treatments except for the positive control which gave 0% viability. The sample is negative for toxicity. Citrinin is known to give an antimicrobial potency to M. purpureus colorants so presence of antimicrobial activity was tested using the Kirby-Bauer test. No zone of inhibition was observed for the various colorant concentrations tested, suggesting the possible absence of citrinin, Mutagenic capacity of the colorant was studied using the micronucleus test. The number of micronucleated polychromatic erythrocytes (MNPCE) out of 1000 polychromatic erythrocytes (PCE) was determined. The data showed a large difference between the mean ratio of MNPCE to PCE for the positive control and the mean ratio of MNPCE to PCE for the colorant samples and the spontaneous mutants group. Duncan's multiple range test also showed no significant differences between the mutagenicity of the colorant and spontaneous mutants indicating that the colorant sample is negative for mutagenicity. Thus, it can be concluded that the colorant is safe since no toxic effects were detected.

Language

English

Location

UPLB Main Library Special Collections Section

Call Number

Thesis

Document Type

Thesis

This document is currently not available here.

Share

COinS