Molecular detection and characterization of canine distemper virus from domestic dogs (Canis familiaris) in Quezon City, Philippines

Date

6-2016

Degree

Doctor of Veterinary Medicine

College

College of Veterinary Medicine (CVM)

Adviser/Committee Chair

Dennis V. Umali

Abstract

Canine Distemper (CD) is a highly infectious viral diseaseaffecting carnivores. In spite of the availability of commercial modified live vaccines, several studies have reported the occurrence of CD in vaccinated animals worldwide. In this study, six dogs with clinical signs of CD were investigated. Oculonasal swabs were collected and nested reverse transcription polymerase chain reaction (nRT-PCR) was performed. Out of six samples, two were positive for CDV. Nucleotide sequencing of the hypervariable region of the hemagglutinin (H) gene showed that the 2 field strains were closely related (94%) to CDVs from Thailand. Genotypic and subgenotypic analyses revealed that the field CDV strains belong to the Asia-4 lineage which was first reported in 2013 in Thailand. At present, this is the first study to genetically characterize CDV in the Philippines.

Language

English

Location

UPLB College of Veterinary Medicine (CVM)

Call Number

LG 993.5 2016 V4 N63

Document Type

Thesis

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