Molecular detection and characterization of canine distemper virus from domestic dogs (Canis familiaris) in Quezon City, Philippines
Date
6-2016
Degree
Doctor of Veterinary Medicine
College
College of Veterinary Medicine (CVM)
Adviser/Committee Chair
Dennis V. Umali
Abstract
Canine Distemper (CD) is a highly infectious viral diseaseaffecting carnivores. In spite of the availability of commercial modified live vaccines, several studies have reported the occurrence of CD in vaccinated animals worldwide. In this study, six dogs with clinical signs of CD were investigated. Oculonasal swabs were collected and nested reverse transcription polymerase chain reaction (nRT-PCR) was performed. Out of six samples, two were positive for CDV. Nucleotide sequencing of the hypervariable region of the hemagglutinin (H) gene showed that the 2 field strains were closely related (94%) to CDVs from Thailand. Genotypic and subgenotypic analyses revealed that the field CDV strains belong to the Asia-4 lineage which was first reported in 2013 in Thailand. At present, this is the first study to genetically characterize CDV in the Philippines.
Language
English
Location
UPLB College of Veterinary Medicine (CVM)
Call Number
LG 993.5 2016 V4 N63
Recommended Citation
Nobleza, Czarina Kristine D., "Molecular detection and characterization of canine distemper virus from domestic dogs (Canis familiaris) in Quezon City, Philippines" (2016). Undergraduate Theses. 4648.
https://www.ukdr.uplb.edu.ph/etd-undergrad/4648
Document Type
Thesis