Antimicrobial effects of Oregano (Oreganumvulgare) crude leaf extract on experimentally induced epidermal burn wounds in rabbits (Oryctolagus cuniculus)

Date

6-2016

College

College of Veterinary Medicine (CVM)

Adviser/Committee Chair

Marco F. Reyes

Co-adviser

Rey B. Oronan

Abstract

The study was conducted to determine the antimicrobial effects of Oreganum vulgare infusion and juice crude extract in burn wounds. A total of 18 apparently healthy adult rabbits, of both sexes were used in the study. A second degree burn was induced on the shaved dorsum where the burn was induced using a stainless steel metal cylinder. Bacterial samples were taken from six randomly selected animals at days one, three, seven and 14 post burn induction and were inoculated in BAP and MacConkey agar for bacterial culture and identification. Isolated bacteria were streaked in MHA for antibiotic sensitivity test. The study showed that 70% (16/23) of the isolate was Staphylococcus spp. followed by Pseudomonas aeruginosa with 13% (3/23). Bacteria were not isolated in 33% (2/6) samples acquired on day one and an increasing number of bacteria were isolated from day three to 14. Gram positive bacteria were most predominant in the first three days, while Gram negative bacteria follows at seven days post induction. Oregano aqueous crude extract exhibited a minimal zone of inhibition against Staphylococcus spp. only.

Language

English

Location

UPLB College of Veterinary Medicine (CVM)

Call Number

LG 993.5 2016 V4 G34

Document Type

Thesis

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