Preliminary studies on the optimizing of pigment production from Rhodotorula rubra

Date

3-2013

Degree

Bachelor of Science in Biology

Major Course

Major in Microbiology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Dalmacio, Ida F.

Abstract

Microorganisms like Rhodotorula rubra can be a source of both extracellular and intracellular pigment. Because of the long-term detrimental effects caused by synthetic colorants, the demand for the production of non-toxic naturally occurring pigments that can be used as colorants is globally increasing. This study aimed to optimally produce and extract pigment from Rhodotorula rubra by utilizing several parameters. The parameters used in this study include (1) incubation period, (2) inoculum level, and (3) initial pH of the media. The experiment was conducted at the Enzyme Laboratory, BIOTECH UPLB. F-test and Duncan's Multiple Range Test (DMRT) were employed to statistically analyze the values obtained. Using coconut water medium, Rhodotorula rubra had the highest extracellular pigment production under the folllowing conditions, seven (7) days incubation, 15-20% level of inoculum (!~~1.9 x 10^4 cells to ~2.5 X 10^5 cells) and 4.0 tp 7.0 initial pH of the media. For the intracellular pigment, conditions that produced the highest amount of pigment were two to seven days incubation period, 20% level of inoculum (~2.5 X 10^5 cells) and pH 4.0 to 7.5 of initial media.

Language

English

Location

UPLB National Institute of Molecular Biology and Biotechnology

Call Number

LG 993.5 2013 B4 /C32

Document Type

Thesis

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