Preliminary studies on the optimizing of pigment production from Rhodotorula rubra
Date
3-2013
Degree
Bachelor of Science in Biology
Major Course
Major in Microbiology
College
College of Arts and Sciences (CAS)
Adviser/Committee Chair
Dalmacio, Ida F.
Abstract
Microorganisms like Rhodotorula rubra can be a source of both extracellular and intracellular pigment. Because of the long-term detrimental effects caused by synthetic colorants, the demand for the production of non-toxic naturally occurring pigments that can be used as colorants is globally increasing. This study aimed to optimally produce and extract pigment from Rhodotorula rubra by utilizing several parameters. The parameters used in this study include (1) incubation period, (2) inoculum level, and (3) initial pH of the media. The experiment was conducted at the Enzyme Laboratory, BIOTECH UPLB. F-test and Duncan's Multiple Range Test (DMRT) were employed to statistically analyze the values obtained. Using coconut water medium, Rhodotorula rubra had the highest extracellular pigment production under the folllowing conditions, seven (7) days incubation, 15-20% level of inoculum (!~~1.9 x 10^4 cells to ~2.5 X 10^5 cells) and 4.0 tp 7.0 initial pH of the media. For the intracellular pigment, conditions that produced the highest amount of pigment were two to seven days incubation period, 20% level of inoculum (~2.5 X 10^5 cells) and pH 4.0 to 7.5 of initial media.
Language
English
Location
UPLB National Institute of Molecular Biology and Biotechnology
Call Number
LG 993.5 2013 B4 /C32
Recommended Citation
Cabaltera, Nyxa Louise Turalde, "Preliminary studies on the optimizing of pigment production from Rhodotorula rubra" (2013). Undergraduate Theses. 468.
https://www.ukdr.uplb.edu.ph/etd-undergrad/468
Document Type
Thesis