Sex identification of peach-faced lovebirds (Agapornisroseicollis) using morphological and behavioural characteristics and polymerase chain reaction-based detection of chromodomain-helicase- DNA1 (CHD1) binding gene isolated from blood and buccal swabs

Date

6-2016

Degree

Doctor of Veterinary Medicine

College

College of Veterinary Medicine (CVM)

Adviser/Committee Chair

Therese Marie A Collantes

Co-adviser

Juan Carlos T. Gonzales

Abstract

Chromodomain-Helicase-DNA1 (CHD1) binding gene detection is one of the simplest, most widely used molecular assays applied to sexually monomorphic birds. This study compared non-molecular and molecular methods. Four morphological traits (body girth, glabella, head circumference, and wingspan) were assessed behavior and pelvic inlet were subjectively evaluated and blood and buccal swabs were obtained for molecular analysis from 18 captive Agapornis roseicollis. PCR-based detection of CHD1 was successfully obtained in five randomly selected birds using varying blood volumes (3μl, 5μl, 10μl, 15μl and 20μl) subjected to DBS procedure. Comparison of combined morphological percent matches to actual PCR results revealed 61.11% for linear discriminant values, 55.56% for behavior, and 72.22% pelvic inlet size which may present high risk of misidentification. This study provides a minimally invasive molecular assay for sex identification in peach-faced lovebirds, and thus may potentially facilitate in accurate sexing of threatened endemic sexually monomorphic avian species in the Philippines.

Language

English

Location

UPLB College of Veterinary Medicine (CVM)

Call Number

LG 993.5 2016 V4 D39

Document Type

Thesis

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