In vitro study on the inhibitory effects of turmeric (Curcuma longa) extracts on some aerobic bacteria found in epidermal burns

Date

6-2016

Degree

Doctor of Veterinary Medicine

College

College of Veterinary Medicine (CVM)

Adviser/Committee Chair

Marco F. Reyes

Co-adviser

Rey B. Oronan

Abstract

Experimentally induced epidermal burns from 18 apparently healthy rabbits were the sources of the swabs for this study. The bacterial swabs were taken at days one, three, seven and 14 post burn induction. These were inoculated in Blood Agar Plate and McConkey Agar. Isolated organisms were inoculated in Mueller Hinton Agar (MHA). Turmeric (Curcumalonga) infusion and juice were impregnated in a punched out filter paper and incubated. The discs were placed on the MHA equidistant to the others (oxytetracyline, cephalexin, silver sulfadiazine).Six aerobic bacteria were isolated. Staphyloccus sp. was the predominant isolate (16 out of 23). Commensal Gram-positive bacteria were the first to colonize the wound while Gram-negative bacteria colonized the wound seven days post induction. Inhibitory effects of both infusion and juice extract were seen only against the growth of Staphylococcus sp.. Moreover, C. longa extracts were found to have relatively similar size of zones of inhibition (ZOI) to that of silver sulfadiazine. Furthermore, betweenthe twoextracts, infusion has a relatively superior inhibitory effect compared to the juice extract based on the measure of their ZOI.

Language

English

Location

UPLB College of Veterinary Medicine (CVM)

Call Number

LG 993.5 2016 V4 C37

Document Type

Thesis

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