Molecular characterization of infectious bursal disease virus from game fowls in selected areas of CALABARZON, Philippines

Date

6-2016

Degree

Doctor of Veterinary Medicine

College

College of Veterinary Medicine (CVM)

Adviser/Committee Chair

Dennis V. Umali

Restrictions

Restricted: Not available to the general public. Access is available only after consultation with author/thesis adviser and only to those bound by the confidentiality agreement.

Abstract

Infectious Bursal Disease (IBD) is a highly infectious, economically important, and immunosuppressive viral disease of poultry. In this study, five gamefowl farms in CALABARZON with clinical signs suggestive of IBD infection were submitted for necropsy. After the observance of gross lesions associated with IBDV, nested RT-PCR was performed and four out of five samples were confirmed to be positive through the use of gel electrophoresis. Nucleotide sequence analyses revealed that the field strains belong to serotype-1 with deduced amino acid sequences of 222A, 256I, 294I, 242I and 299S at the VP-2 gene, which indicates that the field strains were very virulent. Amino acid sequence similarities demonstrated that the field strains were 97-98% identical to Spanish, South African and Nigerian strains and 86.0- 96.0% similar to vaccine strains. Phylogenetic analysis also showed that the field strains belong to the European-like vvIBDV subgenotype.

Language

English

Location

UPLB College of Veterinary Medicine (CVM)

Call Number

LG 993.5 2016 V4 /C33

Document Type

Thesis

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