Phylogenetic analysis of the hypervariable region of the fusion (F) gene of Newcastle disease virus from broiler chickens in selected areas in Central Luzon, Philippines

Date

6-2016

Degree

Doctor of Veterinary Medicine

College

College of Veterinary Medicine (CVM)

Adviser/Committee Chair

Dennis V. Umali

Restrictions

Restricted: Not available to the general public. Access is available only after consultation with author/thesis adviser and only to those bound by the confidentiality agreement.

Abstract

Five field Newcastle disease virus strains (NDVs) were characterized to facilitate the molecular epidemiological investigation of recent outbreak of Newcastle disease (ND) in the Philippines. Pooled infected tissue samples from broiler chickens from Central Luzon with clinical signs suggestive of ND were used in the study. Records of management and farm history were obtained to characterize the clinical profile of the disease. Nucleotide sequencing and phylogenetic analysis were performed using the hypervariable region of the fusion (F) gene. All suspected cases of ND were positive and were velogenic with a proteolytic cleavage site of 112RRQKR*F117. Phylogenetic analysis of five field NDVs showed that all strains belong to genotype VII. Subgenotypic analysis revealed that all strains were clustered to subgenotypeVIIi. Comparison of nucleotide and amino acid sequences showed that the field NDVs were 98.93-100.00% similar. All strains were epidemiologically related and had 99% similarity with Israeli, Indonesian, and Pakistan strains. Amino acid sequence analysis showed that the field strains had 77.60-80.00% similarity with vaccine strains, B1 and La Sota.

Language

English

Location

UPLB College of Veterinary Medicine (CVM)

Call Number

LG 993.5 2016 V4 /B44

Document Type

Thesis

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