Isolation of aflatoxigenic Aspergillus sp. and correlation of fungal growth and aflatoxin concentration in corn using enzyme-linked immunosorbent assay

Date

6-2016

Degree

Doctor of Veterinary Medicine

College

College of Veterinary Medicine (CVM)

Adviser/Committee Chair

Amadeo A Alcantara

Restrictions

Restricted: Not available to the general public. Access is available only after consultation with author/thesis adviser and only to those bound by the confidentiality agreement.

Abstract

Aflatoxin is a toxic secondary metabolite of Aspergillus sp. that commonly contaminates feeds and causes deleterious effect on animals and humans. The correlation of the growth of aflatoxigenic Aspergillus sp. and the aflatoxin it produces was investigated. Aspergillus sp. colonies were isolated from a corn sample which previously tested positive for aflatoxin, which was identified using UV light screening test, gross and microscopic morphology. Seven serial dilutions of Aspergillus culture were plated in four replicates in order to collect and measure aflatoxin levels at 5-day intervals. Quantification of aflatoxin concentration was done using an ELISA test kit. Fungal growth was determined by measuring the area in pixels occupied by the fungi against the total area of the plate using an image editing software. Statistical analysis of the correlation between the growth of the Aspergillus sp. and the amount of aflatoxin produced yielded inverse linear association. The assay also showed that aflatoxin production was at its peak on dilutions 10-3 to 10-4. The results of this study may be applied to future in vitro studies related to aflatoxicosis in the Philippines.

Language

English

Location

UPLB College of Veterinary Medicine (CVM)

Call Number

LG 993.5 2016 V4 /A58

Document Type

Thesis

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