Parametric study on pretreatment and sachaccarification of Macroalga (Sargassum kushimonte) for hydrolysate production.

Date

10-2009

Degree

Bachelor of Science in Chemical Engineering

College

College of Engineering and Agro-Industrial Technology (CEAT)

Adviser/Committee Chair

Rex B. Demafelis

Co-adviser

Milagrosa R. Martinez-Goss

Committee Member

Jovita L. Movillion, Veronica P. Migo

Abstract

Sargassum kushimonte, a brown alga, was characterized as a feedstock for bioethanol production. In relation with that, potential methods for pretreatment and saccharification were evaluated. Acid, alkaline and enzymatic hydrolyses were carried out with their respective parameters. In algal hydrolyses concentrations of acids and alkaline, reaction time and temperature were varied to determine their effect in the rate of algal hydrolyses. On the other hand, enzymatic loading, temperature and reaction time were tested to assess their effects in enzymatic saccharification. In algal hydrolyses, hydrochloric acid yields the highest reducing sugar of about 32.36 mg using the parameters of 3%v/v, 300 minute reaction time and 100oC reaction temperature. Enzymatic saccharification yields reducing sugar a bit higher than HCl hydrolysis, 35.5623 mg, using 0.5 ml cellulase, 50oC and 3 days. Also, compared to acid hydrolysis, enzymatic saccharification does not produce inhibitors for the fermentation. With that, Sargassum kushimonte can be a promising bioethanol feedstock, especially if optimum conditions in enzymatic saccarification are applied, given that it does not have lignin which reduces the pretreatment cost.

Language

English

Location

UPLB College of Engineering and Agro-Industrial Technology

Document Type

Thesis

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