Date

4-2010

Degree

Bachelor of Science in Chemical Engineering

College

College of Engineering and Agro-Industrial Technology (CEAT)

Adviser/Committee Chair

Rex B. Demafelis

Co-adviser

Bayani M. Espiritu

Committee Member

Jovita L. Movillon

Abstract

The use of Jatropha curcas L. as a feedstock for the production of biodiesel is rapidly growing. The cake after oil extraction is rich in protein and is a potential source of animal feed. However, because of the high toxicity of the cake due to the presence of phorbol esters, the cake was subjected to alkali, ethanol and fermentation treatments to remove or reduce the amount of phorbol esters in the cake. Toxicity studies were conducted on tilapia fingerlings by feeding treated as well as untreated cake. The fish fed with raw Jatropha cake was observed to have reduced appetite and poor alertness as compared to those fed with treated cakes. The fish fed with treated cakes exhibited 0% mortality compared to untreated cake fed fish that had 50% mortality rate. The effect of varying fermentation time was also determined and it was found that 7-day fermentation was the best as it gave a pH of 4.7, lowest among the four fermentation periods of 2, 4, 7 and 14 days. The effect of varying inoculum concentration (3% inoculum vs 10% inoculum) was also evaluated and inoculation with ensiling bacteria at 3% rate proved better than 10%. Fermentation proved essential in giving the cake a desirable taste and producing a nutritionally rich fish feed with 19.63% crude protein content higher than the raw Jatropha cake with 18.34%.

Language

English

LC Subject

Biological Assay--Methods, Jatropha, Biomass energy, Microbiology

Call Number

LG 993.5 2010 E62 D43

Document Type

Thesis

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