Optimization of tissue culture medium for in vitro bulbing of garlic (Allium sativum L.)

Date

6-2016

Degree

Bachelor of Science in Agricultural Biotechnology

College

College of Agriculture and Food Science (CAFS)

Adviser/Committee Chair

Lilian F. Pateña

Restrictions

Restricted: Not available to the general public. Access is available only after consultation with author/thesis adviser and only to those bound by the confidentiality agreement.

Abstract

The study aimed to optimize the PCTCL tissue culture medium and design shoot selection criteria for in vitro bulbing of garlic. The treatments used were Gamborg?s medium (B5) with BAP (B5B), B5 with kinetin (B5K), Linsmaier and Skoog (LS) with BAP (LSB), LS with kinetin (LSK), and the control (B5). Garlic shoots from two accessions, Ilocos White and Iloilo, in Garlic Multiplication Medium (GMM), were subcultured onto the five treatments of Garlic Bulbing Medium (GBM) and incubated under controlled environment at the Plant Cell and Tissue Culture Laboratory (PCTCL), Institute of Plant Breeding (IPB). After two months of growth, the average percentage of bulb occurrence/formation, average bulb diameter, and average weight of the regenerated plants were obtained. Results of the study showed that the five treatments had no significant difference in terms of the three parameters, hence, the existing medium (B5) can be optimized by using any of these treatments. However, though insignificant, better response was observed in LSB compared to the control in terms of bulb occurrence and bulb diameter. Shoot diameter size ranges, 0.8?1.2 mm and 1.3-1.6 mm, had significant difference with 1.7?2.00 mm in terms of percentage of bulb occurrence/formation and bulb diameter. Hence, shoots with diameter within 0.8 mm ? 1.6 mm are suitable for in vitro bulbing. With respect to the response of accessions to the test media, garlic shoots from Iloilo performed better than Ilocos White. In 25 L medium, PhP 380.25 can be saved by using LSB instead of B5. For the bulbing medium to be cost effective, the control medium, Gamborg?s B5 medium with the growth regulator 2-ip, 6(dimethylallyamino)purine, may be replaced with Linsmaier and Skoog (LS) medium and 6-benzylaminopurine (BAP) or kinetin.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

LG 993.5 2016 A127 /V53

Notes

Major in Crop Biotechnology

Document Type

Thesis

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