Microsatellite marker screening for heterozygosity in (Lagt X Wat) F1 and parent palms as genotype reference in genetic linkage mapping in coconut (Cocos nucifera L.)

Date

6-2016

Degree

Bachelor of Science in Agricultural Biotechnology

College

College of Agriculture and Food Science (CAFS)

Adviser/Committee Chair

Haydee F. Galvez

Abstract

The coconut palm (Cocos nucifera L.) has been part of the human civilization and had taken a significant role in human existence for its uses from roots to its fruits. Genetic improvement in coconut has remained a challenging pursuit of plant breeders in spite of the great economic importance of the crop. Genetic linkage mapping for important characters such as copra yield and quality, is among the genomics approaches being explored to address this breeding constraint due to genome complexity and very long generation cycle of coconut, especially the outstanding Tall varieties. The DNA samples of (LAGT x WAT) F1 parental hybrids, LAGT, WAT and CATD coconut varieties were subjected to microsatellite (SSR) analysis to screen for markers that exhibit true heterozygosity in any of the (LAGT x WAT) F1 parental hybrids. Furthermore, the backcross parent CATD should inherit and share an allele of these heterozygous SSR loci with the (LAGT x WAT) F1 parent. This is in order to assume a true backcross segregation of the markers in the CATD x ((LAGT x WAT) F1) mapping population. A total of 11 out of 22 SSR markers screened exhibited true allelic inheritance in (LAGT x WAT) F1 parent. While, only nine (9) of these SSR loci ? SSRs 4-01, 2-01, 8-02, 6-02, Z-01, Z-51, 12-03, 1-03 and 10-01, had an allele shared by both the (LAGT x WAT) F1 parent and the backcross parental genotype, coconut CATD. The nine (9) SSR loci were characterized for their polymorphic information content (PIC) as potential markers for genetic linkage application. These markers and the corresponding progenies of the heterozygote (LAGT x WAT) F1 parent/s are recommended for genetic linkage mapping in coconut using the CATD x ((LAGT x WAT) F1) backcross mapping population.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

LG 993.5 2016 A127 /S23

Notes

Major in Crop Biotechnology

Document Type

Thesis

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