Expression analysis of a mads-box gene, MalMADS2, involved in fruit ripening of banana (Musa acuminata Colla cv. Lakatan)

Date

7-2016

Degree

Bachelor of Science in Agricultural Biotechnology

College

College of Agriculture and Food Science (CAFS)

Adviser/Committee Chair

Eureka Teresa M. Ocampo

Abstract

MADS-box family of genes are known to be involved in the regulation of fruit ripening. In Musa acuminata Colla cv. Lakatan, there were four isoforms of the ripening related MADS-box genes, MalMADS2, identified. This study focused on determining gene expression levels of the four MalMADS2 isoforms during the ripening stages of Lakatan. Total RNA was isolated from banana pulp tissues at different stages of ripening. Complementary DNA (cDNA) was synthesized from the isolated RNA for the expression analysis. Relative expression of the four MalMADS2 isoforms was determined using semi quantitative PCR and real-time PCR. Semi-quantitative PCR showed different levels of expression of the four MalMADS2 isoforms at different stages of ripening. Real-time PCR revealed that MalMADS2.1 and MalMADS2.3 were highly expressed at climacteric and 100% yellow stage. On the other hand, MalMADS2.2a and MalMADS2.2b are downregulated after climacteric. Only MalMADS2.2b was observed with almost 2 fold increase in expression during the post-climacteric stages. This differential expression among the four MalMADS2 isoforms suggests possible regulation of MalMADS2.1 and MalMADS3.2 in the ripening of Lakatan.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

LG 993.5 2016 A127 /R69

Notes

Major in Crop Biotechnology

Document Type

Thesis

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