Morphological and molecular detection of Colletotrichum sp. associated with sampaguita (Jasminum sambac) from Sta. Cruz, Laguna

Date

12-2015

Degree

Bachelor of Science in Agricultural Biotechnology

College

College of Agriculture and Food Science (CAFS)

Adviser/Committee Chair

Johnny F. Balidion

Abstract

The research internship was conducted at the Crop Protection Cluster, College of Agriculture, University of the Philippines Los Baños (UPLB) from April 2015 to December 2015. The activities include the morphological and molecular detection of Colletotrichumsp. associated with sampaguita (Jasminunsambac) collected from Sta. Cruz, Laguna, Philippines. For the morphological identification portion, isolation, pathogenicity testing and reisolation of the suspected pathogen were done as well as the observation of characteristics appearance of the fungal isolate grown on PDA media, mycelial and spores characters. The appearances of the spores produced by the isolates were of two distinct types, namely, oval-shaped and crescent-shaped without septae and its mycelia were branched. For molecular detection, specific primers namely: internal transcribed spacer (ITS)4, ITS5 were used as the universal primer β-tubulin (Bt)2a, Bt2b Gs-R, Gs-F and Ap-Mat(Am)-R, Am-F were used for the detection of Colletotrichumsp. Furthermore DNA elongation factor primers(EF)1, EF2 were used in the detection of Fusariumsp..Results showed that the fungal isolates from sampaguita belonged to the genus Colletotrichumbased on its morphological characters and the presence of the amplified PCR products using the primers. In addition, no amplicons were observed using EF1 and EF2 primers which confirm that the isolates with C shaped conidia are not members of the genus Fusariumbut Colletrotrichum.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

LG 993 2015 A127 /P53

Notes

Major in Crop Protection Biotechnology

Document Type

Thesis

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