Assessment of genetic diversity in stingless bees, Tetragonula spp. (Hymenoptera: Apidae), from Batangas, Cavite, and Laguna using isozyme analysis

Date

4-2013

Degree

Bachelor of Science in Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Dela Vina, Celia B.

Co-adviser

Dela Vina, Celia B.

Committee Member

Cadiz, Nina M.

Abstract

Genetic diversity in populations of Tetragonula spp. from Batangas, Cavite and Laguna was assessed using isozyme analysis. Nine presumptive loci were observed for four enzymes studied. There were three loci each for acid phosphatase (ACP) and esterase (EST), one locus for malate dehydrogenase (MDH), and two loci for malic enzyme (ME). Slow (S) and fast (F) alleles were noted for all other loci except ME-1 which was monomorphic and found only in the samples from Cavite. The loci MDH-1, ME-2, EST-2, ACP-1 and ACP-3 were polymorphic in Batangas population while MDH- 1, ME-2, EST-1 and ACP-1 were polymorphic in Laguna population. On the other hand, EST-1, EST-2, EST-3 and ACP-2 were found to be polymorphic in Cavite population. The intrapopulation variation was assessed by computing for the proportion of polymorphic loci (P), average number of alleles per locus (A) and mean heterozygosity (He). P, A and He were highest in the Batangas population with values of 0.556 for P, 1.625 for A and 0.350 for He. The observed values indicate that the Batangas population had the highest genetic variability compared to the Laguna and Cavite populations. Cavite population had the lowest A (1.444) and He (0.175) suggesting that this population had the lowest genetic variability. Genetic identity (IN), genotypic identity (IH) and genetic distance (D) were computed to measure the interpopulation variation. Batangas and Laguna populations had the highest degree of genetic identity (0.914) and lowest genetic distance (0.090) while Batangas and Cavite populations had the lowest degree of genetic identity (0.822) and the highest genetic distance (0.196). Genotypic similarity was highest between Batangas and Cavite populations (0.411), followed by Batangas and Laguna populations (0.296) a nd was least similar between Laguna and Cavite populations (0.242). In general, the computed estimates suggest the highest genetic relatedness between the Batangas and Laguna populations and the least between the Batangas and Cavite population. The observed variation among populations may be influenced by migration patterns and other environmental factors.

Language

English

Location

UPLB Main Library Special Collections Section

Call Number

LG 993.5 2013 B4 N38

Document Type

Thesis

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